Figure 1.—

Complementation of Scpho5Δ by C. glabrata genomic library. (A) A semiquantitative phosphatase plate assay was performed on wild-type C. glabrata grown in high-phosphate media and in no-phosphate media with the dark color indicating secreted acid phosphatase activity. Five genomic clones conferred phosphatase activity in no-phosphate conditions and repressed phosphatase activity in high-phosphate conditions. (B) The genomic clones with phosphatase activity were further analyzed. Clone A spans nucleotides 740743–747471, clone E 748278–738994, clone Q 747445–735219, and clone T 743041–747455. Clone O ends at nucleotide 747642 (numbering is based on chromosome K sequence NC_006034). Using CgPMU1 primers designed to amplify the ORF, it was determined that clone O contains CgPMU1; however, one end was not refractory to sequencing. The same region of chromosome 11 from S. cerevisiae is below the C. glabrata schematic showing the conserved synteny. The direction of the arrows indicates the direction of transcription.