Figure 4.—

CgPMU2 is strongly regulated by phosphate starvation conditions and the transcription factor Pho4. Quantitative real-time PCR was performed on each PMU gene and normalized to the expression level of ACT1. Each primer set was verified to amplify only the indicated gene by examining qPCR of deletion strains (Figure S3). CgPMU1 and CgPMU2 appear regulated by CgPho4, but CgPMU1 to a much lesser extent. The CgPMU3/CgACT1 ratio is ∼0.003 and does not change in response to phosphate condition or CgPHO4 deletion.