Figure 5.—

The CgPMU2 and CgPMU3 ORFs are able to hydrolyze 1-NP or PNPP efficiently. All four ORFs were placed under the control of the phosphate starvation-regulated ScPHO5 promoter and transformed into Cgpmu2Δ strain. Expression of each ORF was verified by RT–qPCR (Figure S4). Phosphatase activity is restored when CgPMU2 and CgPMU3 are expressed as judged by hydrolysis of 1-NP (A) or PNPP (B). Additionally, these ORFs were placed under the control of the CgPMU2 promoter and exhibited similar results (Figure S5). Multiple isolates were subjected to the phosphatase plate assay and these are representative results.