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CgPMU2 is required for the growth of C. glabrata in media with organic phosphate as the sole phosphate source. Strains were inoculated at OD600 of 0.001 and grown for 20 hr in SD + 0.2 mm phosphate or 72 hr in SD + organic phosphate. GMP, guanosine monophosphate; G2P, glycerol-2-phosphate; Pi, inorganic phosphate. Strains grew much slower in organic phosphate sources and 500 μm G2P was required for measurable growth. This is one representative experiment, but was reproducible.
