TABLE 2.
Activity of His10-tagged purified proteins (μmol phosphate released/min × mg protein) against various phosphate-containing compounds measured in millimolar concentrations
| Pmu1 |
Pmu2 |
Pmu3 |
||||
|---|---|---|---|---|---|---|
| Substrate | Km | Vmax | Km | Vmax | Km | Vmax |
| PNPP | 1.7 ± 0.83 | 2.6 ± 0.39 | 0.81 ± 0.03 | 46 ± 6.1 | 1.7 ± 0.2 | 2.2 ± 0.16 |
| Glycerol-1-phosphate | 24 ± 0.03 | 29 ± 2.2 | 2.2 ± 0.64 | 11 ± 1.0 | 98 ± 30 | 42 ± 8.0 |
| Glycerol-2-phosphate | 23 ± 3.3 | 29 ± 5.7 | 25 ± 11 | 14 ± 3.3 | ND | ND |
| IMP | ND | ND | 6.4 ± 3.4 | 11 ± 0.87 | ND | ND |
| Trehalose-6-phosphate | 0.26 ± 0.001 | 0.29 ± 0.006 | ND | ND | 1.1 ± 0.38 | 0.24 ± 0.08 |
ND, no activity detected.