Figure 7.—

Coffee extract exposure protects against GFP∷degron toxicity in a skn-1–dependent manner. (A) CL2337 (Pmyo-3∷GFP∷degron) worms were propagated on control or 10% decaffeinated coffee plates seeded with E. coli expressing control (vector only) or skn-1 dsRNA. Induction of GFP∷degron expression also results in paralysis (Link et al. 2006). Coffee extract exposure produces strong protection in worms propagated on the control RNAi strain (black vs. red lines, P < 0.001, paired log rank survival test), but not in worms propagated on skn-1 RNAi (blue vs. orange lines). (B) Fused DIC/epifluorescence image of GFP∷degron worms propagated on control or 10% decaffeinated coffee plates. Distribution or size of the GFP∷degron deposits was not detectably altered by coffee exposure. Bar, 20 μm. (C) Immunoblot assaying GFP∷degron accumulation in worms propagated on control or 10% decaffeinated coffee plates. Equal gel loading was confirmed by reprobing the blot with antibody against CstF64, a slicing factor unlikely to be influenced by coffee exposure.