Figure 1.—

Inducing β1 integrin deletion in smooth muscle cells. (A) Schematic representation of the location of the loxP sites in the β1^fl and β1^e3 transgenic mice (Potocnik et al. 2000; Raghavan et al. 2000). (B) Distribution of genotypes and viability of genetic crosses between cβ1^+/fl mice and β1^fl/fl mice. (C) Histology of β-gal–stained bladders from iβ1^fl/fl and iR26R either 24 hr or 48 hr postdose (PD) at indicated doses. Arrows denoted β-gal positive cells. (D) Histology of β-gal–stained bladders from iβ1^fl/fl and iR26R mice exposed to 1 mg tamoxifen daily for 28 days. Arrows point to cells negative for β-gal. (E) Western blot from bladder lysates of 28 × 1 mg iβ1^fl/fl, oil-injected iβ1^fl/fl, with cβ1^fl/fl as a positive control and cβ1^+/fl as a negative control for protein loss and α-tubulin as a loading control.