Figure 3.—

Identification of high-copy suppressors of synthetic lethality in spo7Δ mps3 mutants. spo7Δ mps3-1 cells maintained with the pURA3-MPS3 plasmid (KW228) were transformed with the indicated suppressor plasmids (clones 62 and 146), an empty vector, and constructs containing full-length (-FL) or truncated forms (-T) of NUP157 and NIC96 cloned behind the TEF promoter. Each construct was tested by serial dilution for the ability to restore growth at 23° on plates containing 5-FOA or control (SC) media.