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. 2001 Mar 15;29(6):1300–1307. doi: 10.1093/nar/29.6.1300

Figure 5.

Figure 5

DNA DSBs prevent hPTTG–Ku-70 association. (A) NP-40 lysates from HL-60 cells (5 × 106) were preincubated with sonicated salmon sperm DNA (SS), pUC18 digested with EcoRI (LP) or supercoiled pUC18 DNA (SD) at 100 µg/ml or with the buffer alone (–) prior to incubate with 6His-hPTTG. Western blots were developed with anti-Ku-70. HL-60, extract from 5 × 105 HL-60 cells. (B) Similar experiments were performed but using 200 µg/ml of sonicated salmon sperm DNA (SS). 6His-hPTTG-HXh was used as control. HL-60, extract from 5 × 105 HL-60 cells. (C) NP-40 lysates from HL-60 cells (5 × 106) were incubated with 6His-hPTTG. Resin was washed in lysis buffer and then incubated with sonicated salmon sperm DNA (SS) at 100 µg/ml or with the buffer alone (–). Western blots were developed with anti-Ku-70. HL-60, extract from 5 × 105 HL-60 cells.