Figure 1.—

Locus-specific regulation of silent chromatin by R95 and R102 mutants. (A) Telomeric silencing of the URA3 reporter at telomere VIIR was measured by growth on synthetic medium lacking uracil (SC−Ura) or SC medium containing 5-FOA (SC +5-FOA). Silencing of the ADE2 reporter at telomere VL was measured by colony color. Strains tested only contain a single copy of either the wild-type or the indicated mutant histone H2A and histone H2B genes. (B) Telomeric silencing was measured as in A, but the strains contained intact histone genes with an additional copy of the mutant genes. (C) rDNA silencing of URA3 reporter was measured by growth of cells on SC +5-FOA. (D) rDNA silencing of the MET15 reporter was measured by color of colonies on MLA plates.