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. Author manuscript; available in PMC: 2011 Oct 21.
Published in final edited form as: Soft Matter. 2010 Oct 21;6(20):5127–5137. doi: 10.1039/c0sm00173b

Figure 2.

Figure 2

Over-proliferation of transplanted ESNPCs. A subset of transplanted ESNPCs over-proliferated damaging uninjured spinal cord axons and cause a dramatic decrease in behavioral function. A) Over-proliferation was characterized by growth of ESNPCs that expanded from the ventral to the dorsal surface of the spinal cord. The image shows a sagittal section of a spinal cord with ESNPCs over-proliferation. The section was stained with Tuj1 (neurons, red), GFAP (astrocytes, blue) and GFP (ESNPCs, green). B) The proliferation marker Ki67 was used to visualize proliferating cells at 4 and 8 weeks after transplantation. The vast majority of Ki67 staining (blue) is co-localized with SSEA-1 (red) staining. The staining suggests that SSEA-1 positive mouse embryonic stem cells are contributing to the over-proliferation.