Fig. 1.
Analysis of genome-wide in vivo RNAi screen. (A) Total data of all RNAi lines screened for survival after S. marcescens infections. Data were analyzed as the time, in days, when 50% of the total number of flies had died. All data were normalized to the daily LT50 mean of an experimental cohort. In all experiments the cohort ranged from 80-200 lines. Hits were defined by susceptible (red dashed line) and resistant (blue dashed line) cut-offs, i.e. 1.5 standard deviations (SD) below the mean and 2 SD above the mean, respectively, based on the pilot screen and controls. (B). Effect of RNAi knock-down of IMD and Toll pathway components on their survival against S. marcescens infection. SCOREs are shown for each line as described in Methods. The dashed lines indicate the cut-offs used for resistance (+2 SD) and susceptibility (−1.5. SD) candidates. (C) Percentage distribution of gene ontology (GO) annotated genes to biological processes for susceptible candidates.