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. 2010 Nov 2;1(5):e00190-10. doi: 10.1128/mBio.00190-10

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Copyright © 2010 Flynn et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 2 — Glycerol utilization and ethanol production in engineered S. oneidensis strains. (A) Plasmid map of pGUT2PET. (B) Aerobic growth of S. oneidensis with pGUT2PET (solid triangles), S. oneidensis with an empty vector (solid squares), and E. coli K-12 (solid circles) on glycerol. Anaerobic resting cell fumarate batch reactions with the wild-type strain with pGUT2PET (C) and the ∆pta mutant with pGUT2PET (D). (C and D) Glycerol (green line and solid circles), ethanol (blue line and solid triangles), and acetate (black line and solid squares) concentrations were determined by analyzing culture supernatants. Error bars represent standard deviations of results from at least three independent experiments.