TABLE 2 .
Bacterial strains, vectors, and primers used in this study
| Strain, vector, or gene and primera | Relevant characteristic(s) | Reference(s) or source |
|---|---|---|
| Strains | ||
| S. oneidensis | ||
| MR-1 | Isolated from Lake Oneida, NY | 17, 48 |
| JG612 | ∆pta deletion derivative of MR-1 | 27 |
| E. coli | ||
| K-12 | Laboratory stock | |
| UQ950 | E. coli DH5α for cloning | 57 |
| WM3064 | DAP auxotroph donor strain for conjugationb | 57 |
| Vectors | ||
| pBBR1MCS-2 | 5.0-kb broad-host-range vector for cloning; Kmr | 51 |
| pPET | pBBR1MCS-2 containing pdc and adh (cloned from Zymomonas mobilis, pLOI297) | This study |
| pGUT2 | pBBR1MCS-2 containing glpD, glpF, glpK, and tpiA (cloned from E. coli K-12) | This study |
| pGUT2PET | pBBR1MCS-2 containing glpD, glpF, glpK, and tpiA (cloned from E. coli K-12) and pdc and adh (cloned from Z. mobilis, pLOI297) | This study |
| Primers for cloning | ||
| glpD | ||
| J1 KpnI | GGGGTACCACGAAAGTGAATGAGGGCAGCA | This study |
| J2 XhoI | CCGCTCGAGCAGGCCAGATTGAAATCTGA | |
| glpFK | ||
| J3 XbaI | GCTCTAGAAGCATGCCTACAAGCATCGTG | This study |
| J4 NotI | ATAAGAATCGGGCCGCTGCGGCATAAACGCTTCATTCG | |
| tpiA | ||
| J5 SacI | NNGAGCTCCGCTTATAAGCGTGGAGA | This study |
| J6 SacI | NNGAGCTCGAAAGTAAGTGCCGGATATG | |
| glpABC | ||
| J7 HindIII | CCCAAGCTTGCGCGAAATCAAACAATTCA | This study |
| J8 EcoRI | CGGAATTCATACATTGGGCACGGAATCG | |
| pUCmod Fwd | ||
| J9 XhoI | NNCTCGAGCCCGACTGGAAAGCGC | This study |
| pUCmod Rev | ||
| J10 SacI | NNNGAGCTCACATGCGGTGTGAAATACCG | This study |
| pBBR1MCS-2 Rev | ||
| J11 XhoI | NNNCTCGAGCTCTAGAACTAGTGGATCCC | This study |
a
Fwd, forward; Rev, reverse.
b
DAP, diaminopimelic acid.