FIG 5 .

Mutation of the ICP27 zinc finger affects the functional interaction of ICP27 and Hsc70. (A) RSF were either mock infected or infected with HSV-1 KOS or 27-LacZ as indicated for 6 h. Cells were fixed and stained with anti-Hsc70 antibody as described (5). (B, C) RSF were cotransfected with N-Venus–Hsc70 and ICP27–C-Venus (B) or with ICP27-C483,488S–C-Venus (C) as indicated and infected with 27-LacZ. At 8 h after infection, Venus fluorescence was viewed using a Zeiss LSM 510 Meta confocal microscope at a magnification of ×63. White arrows point to Hsc70 nuclear foci.