Figure 1.

Southern blot analysis of a MAGGY transformant of M.grisea (R0) and its protoplast regenerants (R1). (A) Restriction map of MAGGY and the ES fragment used for probing the Southern blots. (B) Southern blot of DNA samples that were extracted from R0 and R1 mycelia, digested with EcoRI and probed with the ES fragment. Arrowheads, MAGGY bands presumed to have already occurred in the isolated single protoplast; circles, MAGGY bands presumed to have appeared during the culture of mycelia after the protoplast isolation (see text). E, EcoRI; S, SmaI; X, XhoI.