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. 2001 Mar 15;29(6):1278–1284. doi: 10.1093/nar/29.6.1278

Figure 5.

Figure 5

Restriction endonuclease analysis to determine methylation status of MAGGY (A) and MGR583 (B) sequences in M.grisea isolates from various monocot plants. Genomic DNA was extracted from mycelia, digested with isoschizomers HapII (H) and Msp I (M), and hybridized with a 5.4 kb XhoI fragment of MAGGY. HapII is sensitive to cytosine methylation but MspI is not. Southern blots of isolates 7, 8 and 9 were overexposed in (A) due to the low copy numbers of MAGGY-related sequences in them. 1, IN77-16-1-1 (isolated from foxtail millet in India); 2, NNSI2-1-1 (foxtail millet in Japan); 3, GFSI1-7-2 (foxtail millet in Japan); 4, IN77-46-1-3 (rice in India); 5, 1601-3 (rice in Japan); 6, 1836-3 (rice in Japan); 7, IN77-28-1-1 (P.repens in India); 8, STPM4-3-3 (P.miliaceum in Japan); 9, YNPM2-2-1 (P.miliaceum in Japan).