A, Representative images showing FM1–43 dye loading in 3rd instar larval NMJs loaded in depolarizing saline for 4 minutes at 18°C. Scale bar, 2µm. Lower panels show higher magnification of synaptic boutons from terminals in above panels. Scale bar, 1µm. B, Quantification of FM1–43 dye loading showing relative dye loading intensity between OR, shits1/y, and shits1/y; rbots/Δ genotypes. Significance of p<0.01 is indicated (**).