Figure 3.
Latent viral DNA loads in wt and p35-/- mice. TG were removed from C57BL/6 and p35-/- latently infected mice at 30 days p.i., and DNA was isolated from each TG. The relative establishment of HSV-1 latency for each mouse strain (n = 14 per group) was determined by real-time PCR using primers that amplified the HSV-1 UL50 gene. UL50 gene levels were normalized to a DNA loading control, the mouse adipsin gene. Data shown are logarithmic (base 2) means. Error bars represent SEMs. Mockinfected samples were below the limit of detection for the UL50 gene (data not shown).