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Effect of HAP1 upon the Fapy-DNA glycosylase activity of hOGG1. The substrate (2 pmol) was incubated with 250 fmol of hOGG1 for 90 min in the presence of 2.5 pmol of either HAP1, HAP1-D210N or endo IV. The [3H]Me-FapyG excision products were quantified in a scintillation counter. Mean values and standard deviations were calculated from four separate experiments.
