Skip to main content
. 2001 Mar 15;29(6):1285–1292. doi: 10.1093/nar/29.6.1285

Figure 5.

Figure 5

HAP1 effect on the hOGG1–AP site complex. 5′-End-labelled 34mer duplex containing a abasic analogue site (F) (10 fmol) was pre-incubated with an excess of GST–OGG1 protein (215 fmol) to form the GST–OGG1–AP site complex (time 0). After 5, 15 and 30 min incubation at 37°C in the presence or absence of HAP1 or its mutant form (250 fmol), samples were loaded onto a pre-running non-denaturing polyacrylamide gel to separate the protein–DNA complexes from the free DNA. The positions of free DNA, HAP1–DNA and GST–OGG1–DNA complexes are shown by arrows.