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Binding of HAP1 protein to a duplex DNA containing an AP site or a 5′ incised AP site. Binding reaction mixtures containing 0, 25, 125, 250 and 500 fmol of hOGG1 protein and 5′-32P-labelled [F:C] (AP site) or endo IV-treated [F:C] (incised AP site) duplexes were carried out as indicated in Materials and Methods. The positions of free DNA and hOGG1–DNA complexes are shown by arrows.
