Table 1. Kinetic parameters for the dissociation of the hOGG1–[F:C] complex.

	–HAP1	+HAP1
koff	5.00 × 10–3 s–1	4.83 × 10–3 s–1
Half-life of the complex	2.3 min	2.4 min

The amount of protein–DNA complex as a function of time was determined using EMSA (see Materials and Methods) and the kinetic parameters were derived by fitting the data to an exponential curve.