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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Free Radic Biol Med. 2010 Aug 6;49(9):1406–1416. doi: 10.1016/j.freeradbiomed.2010.07.026

Figure 1. CYP2E1 activities and expression in WT, CYP2E1 knockout and humanized CYP2E1 knockin mice fed dextrose or ethanol diets.

Figure 1

A. Western blotting analysis for CYP2E1 expression. A typical immunoblot is shown and CYP2E1/actin ratios from 3–6 pairs of mice in each group are indicated. * P <0.05 compared with dextrose group.

B. Microsomal PNP activities. ** P<0.01, compared with WT dextrose (WD) group; ## P<0.01, compared with KI dextrose (KID) group; && P<0.01, compared with WT ethanol (WE) group. KOD, CYP2E1 knockout mice fed dextrose diet; KOE, CYP2E1 knockout mice fed ethanol diet; KIE, humanized CYP2E1 knockin mice fed ethanol diet. (n= 3–6 pairs of mice in each group).

C. Immunohistochemistry staining to determine CYP2E1 in the liver.

D. TNFα levels in liver lysates were analyzed by an ELISA method. (n= 3–6 pairs of mice in each group).