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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Immunol. 2010 Oct 8;185(9):4988–4992. doi: 10.4049/jimmunol.1002042

Figure 3. Defective bone marrow localization and proliferation Eomes KO memory CD8+ T cells.

Figure 3

(A) CXCR4, integrin α4, and integrin β1 expression on CD8+ GP33+T cells from spleens of Eomes KO/WT bone marrow chimeras 60 days after LCMV infection. Plots are representative results from three chimeric mice.

(B) Quantitative RT-PCR of CXCR4 or CXCR3 mRNA from wild-type versus Eomes KO central-memory (CD44hi CD62Lhi) CD8+ cells sorted from an individual Eomes KO/WT bone marrow chimera 60 days after infection with LCMV. Data are representative of two independent experiments.

(C) BrdU uptake in Eomes KO versus wild-type CD8+ GP33+ T cells from the bone marrow of Eomes KO/WT bone marrow chimeras 60 days after LCMV infection. Data are derived from four chimeric animals.

(D) Quantitative RT-PCR of Bcl-2 mRNA as in (B).

(E) CD122, CD127, and CD27 expression on CD8+ GP33+ T cells from spleens of wild-type and Eomes KO mice 60 days after infection with LCMV. Data are representative of three independent experiments.