(A) CXCR4, integrin α4, and integrin β1 expression on CD8+ GP33+T cells from spleens of Eomes KO/WT bone marrow chimeras 60 days after LCMV infection. Plots are representative results from three chimeric mice.
(B) Quantitative RT-PCR of CXCR4 or CXCR3 mRNA from wild-type versus Eomes KO central-memory (CD44hi CD62Lhi) CD8+ cells sorted from an individual Eomes KO/WT bone marrow chimera 60 days after infection with LCMV. Data are representative of two independent experiments.
(C) BrdU uptake in Eomes KO versus wild-type CD8+ GP33+ T cells from the bone marrow of Eomes KO/WT bone marrow chimeras 60 days after LCMV infection. Data are derived from four chimeric animals.
(D) Quantitative RT-PCR of Bcl-2 mRNA as in (B).
(E) CD122, CD127, and CD27 expression on CD8+ GP33+ T cells from spleens of wild-type and Eomes KO mice 60 days after infection with LCMV. Data are representative of three independent experiments.