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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cancer. 2010 Nov 1;116(21):4980–4990. doi: 10.1002/cncr.25426

FIGURE 6.

FIGURE 6

Dasatinib-induced autophagy partially depends on Bcl-2. (A), Effect of dasatinib on Bcl-2 protein as detected by Western blotting. HEY cells were treated with dasatinib for 24 hrs. (B), Effect of dasatinib on Bcl-2 mRNA as detected by quantitative PCR. HEY cells were treated with dasatinib for 24 hrs. *, p < 0.05. (C), Effect of dasatinib on Bcl-2 promoter activity as detected by luciferase reporter assay. Relative luminescence units (RLU) were normalized with protein concentrations in each samples and final values of RLU were expressed as RLU per µg protein per ml. (D), Effect of Bcl-2 overexpession on dasatinib-induced autophagy as detected by AO staining. HEY Bcl-2 stable subclones #26 and #27 were treated with dasatinib for 24 hrs. AO staining was measured with flow cytometry. *, p < 0.05. (F), Effect of Bcl-2 overexpession on dasatinib-induced autophagy as determined by GFP-LC3 fluorescence microscopy. Bcl-2 stable sublines #26 and #27 were transiently transfected with pGFP-LC3 and then treated with dasatinib for another 24 hrs. *, p < 0.05. **, p < 0.01.