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. Author manuscript; available in PMC: 2010 Nov 8.
Published in final edited form as: Development. 1991 Jun;112(2):439–449. doi: 10.1242/dev.112.2.439

Fig. 7.

Fig. 7

Characterization of the gelatinases. Mammary tissue extracts from 13-day pregnant (pl3), 9-day lactating (L9), and 2- and 4-day involuting (i2 and i4 respectively) mammary gland were separated on SDS–gelatin gels and then incubated without (A) or with (B) TIMP at 10 μg ml−1 in substrate buffer. (C) Activation of the 68K gelatinase after incubation of tissue extract with APMA. L9 and i4 tissue extracts were incubated for 16 h at ambient temperature in 1 mM APMA before loading on the gel. Lanes were loaded with 15 μg of protein.