Figure 4.

Chromosomal DNA cleavage stimulated in heavily infected macrophages by IFN-γ requires Stat1 but is independent of TNF-α or IRF-1. BMDM from TNF-α KO A) IRF-1 KO B) Stat1 KO C) and wild type (WT) mice were pretreated with IFN-γ overnight and infected with BCG at MOI of 25 for 9 h. Cells were then lysed and oligonucleosomes quantified by antigen-capture ELISA. Data are expressed as mean fold increase in oligonucleosomes ± SEM of three independent experiments. *p < 0.05; **p < 0.01.