Table 1.
Data collection and processing:
| Crystal design | Single triangle (Nature 2009 461, 74–77.) | Two triangles (A+B)1 (RCSB code 3NAO) |
| Unit cell (R3) | a = 69.22 Å, α = 101.44° (a = 87.65 Å, α = 75.37°)2 | a = 86.36 Å, α = 75.16° (a = 68.31 Å, α = 101.59°)3 |
| Volume (Å3) of unit cell/ASU4 | 308708/102903 | 589184/196395 |
| Content of unit cell/ASU | One triangle/1/3 triangle | Two triangles (A+B)/1/3 each of triangles A and B |
| Resolution (Å) | 17.3-4.01 | 35.0-5.0 |
| Wavelength (Å) | 1.0 | 0.984 |
| Redundancy5 | 7.3(6.0) | 9.0(4.5) |
| Completeness (%)5 | 96.1(100.0) | 97.2(87.1) |
| Rmerge5 | 0.067(0.594) | 0.056(0.461) |
| I/σ | 10.9 | 15.2 |
| Refinement statistics: | ||
| Resolution (Å) | 35.0-5.0 | |
| Rwork/Rfree | 0.1821/0.2393 | |
| Number of nucleic acid atoms | 1710 | |
| Number of non-nucleic acid atoms | 0 | |
| R.m.s.d. of angle and bond | 1.221°/0.0052Å | |
1
Data collected at beamline X6A at NSLS;
2
This unit cell of double triangles design is derived from the unit cell of single triangle design;
3
This unit cell of single triangle design is derived from the unit cell of double triangles design;
4
ASU = asymmetric unit;
5
Numbers in parenthesis represent values for the highest resolution bin