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. 2001 Mar 15;29(6):1272–1277. doi: 10.1093/nar/29.6.1272

Figure 2.

Figure 2

Alignment output from Gene2EST with features subsequently colour-coded for clarity. The query is a fragment of the human aldose reductase gene (EMBL accession no. AF032455) spanning exons 4 and 5. To save space, only a few of the HSPs from matching ESTs are shown. The intron contains a class Y Alu insertion (blue). Splice donor (red) and acceptor (green) signals abut the exon borders. Where possible, the parser has trimmed HSP overlaps to the GT/AG splice boundaries: pink bases show HSP ends that could not be trimmed by the parser, as there is no adjacent exon within the given EST.