Figure 3.
A summary of averaged hydrophobicity (KD) from peptides identified in six proteolytic conditions. The Kyte-Doolittle hydropathy score (KD) of peptides identified in each digestion condition was calculated and plotted in histograms. The average KD of peptides from each digestion condition is summarized in a table. The result shows that aqueous digestions in combination with the MS-compatible detergents yield more hydrophobic peptides (lower negative KD value) than organic solvent digestions.