Table 1.
Categorization of proteins identified in 6 different trypsin digestion conditions with MS compatible detergents based on the cellular localization of Gene Ontology (GO).
| ACN IVS | ACN PPS | ACN RPG | Tris IVS | Tris PPS | Tris RPG | ||
|---|---|---|---|---|---|---|---|
| Predicted TM (%) | 423 (16.0) | 466 (16.9) | 450 (16.3) | 476 (17.8) | 431 (15.0) | 556 (16.9) | |
| No Predicted TM | |||||||
| Integral to membrane | 6 | 4 | 4 | 7 | 8 | 10 | |
| Plasma membrane | 30 | 27 | 26 | 29 | 27 | 34 | |
| Mitochondrial membrane | 27 | 30 | 33 | 33 | 32 | 37 | |
| Nuclear membrane | 18 | 14 | 17 | 15 | 6 | 8 | |
| Golgi membrane | 7 | 15 | 9 | 7 | 9 | 9 | |
| ER membrane | 6 | 10 | 5 | 6 | 5 | 9 | |
| Lysome membrane | 1 | 1 | 1 | 1 | 1 | 1 | |
| Membrane | 128 | 140 | 142 | 139 | 151 | 162 | |
| Cytoskeleton | 120 | 120 | 114 | 119 | 116 | 128 | |
| Cytoplasmic | 344 | 319 | 335 | 318 | 362 | 381 | |
| Nucleus | 162 | 168 | 173 | 157 | 286 | 204 | |
| Mitochondrion | 84 | 95 | 85 | 82 | 84 | 96 | |
| ER | 21 | 23 | 23 | 21 | 25 | 26 | |
| Endosome | 4 | 5 | 5 | 5 | 3 | 4 | |
| Lysosome | 4 | 6 | 5 | 4 | 5 | 6 | |
| Microsome | 2 | 2 | 2 | 2 | 2 | 3 | |
| Peroxisome | 2 | 4 | 5 | 4 | 4 | 6 | |
| Extracellular | 29 | 30 | 33 | 20 | 25 | 25 | |
| No assignment | 1227 | 1278 | 1302 | 1229 | 1295 | 1586 | |
| Total Protein (Spectra) Identified | 2645 (23779) | 2757 (22902) | 2769 (21873) | 2674 (22237) | 2877 (19642) | 3291 (25338) |
*
TM = transmembrane