Skip to main content
. 2010 Nov 8;5(11):e13871. doi: 10.1371/journal.pone.0013871

Figure 3. Complementation of the S. Typhimurium ΔsucAB strain in resting RAW macrophages and cytotoxicity assays.

Figure 3

(A) Numbers of viable bacteria (expressed as percentages of the initial inoculum) inside the macrophages at 2 h and 18 h after infection. Each bar indicates the statistical mean for three biological replicates, and the error bars indicate the standard deviations. The significant differences between the parental 4/74 strain and the mutant and complemented strains are shown by asterisks p>0.05, * p<0.05, ** p<0.01, and *** p<0.001. (B) Cytotoxicity assays of S. Typhimurium wild-type, ΔsucCD (AT3449) and complemented ΔsucCD (AT???) strains in RAW macrophages after 18 h infection as a percentage of total LDH release from lysed uninfected macrophages. All cytotoxicity data were obtained from three independent biological replicates.