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. 2010 Nov 8;5(11):e13871. doi: 10.1371/journal.pone.0013871

Figure 5. Decreased intracellular replication of S. Typhimurium ΔsucCD and ΔgltA strains during infection of HeLa epithelial cells.

Figure 5

Intracellular replication assays of S. Typhimurium 4/74, ΔsucCD (AT3449), ΔgltA (AT3505) and Δmdh (AT3508) strains during infection of HeLa cells. The data show the number of viable bacteria (expressed as percentages of the initial inocula) within macrophages at 2 h and 6 h post-infection. Since S. Typhimurium initiates intracellular replication much earlier in HeLa cells (3–4 h) compared to macrophages (∼8 h post-infection) replication was assessed at 6 h post-infection (Hautefort et al., 2008). Each bar represent the statistical mean from three independent biological replicates and the error bars represent the standard deviation (The significant differences between the parental 4/74 strain and the mutant strains are shown by asterisks p>0.05, * p<0.05, ** p<0.01, and *** p<0.001).