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. 2010 Nov 8;5(11):e13871. doi: 10.1371/journal.pone.0013871

Table 1. Primers used to construct S. Typhimurium gene deletion mutants.

Primer Name Primer DNA sequence
aceAredf CTGGCTTAATTCACCACATAACAATATGGAGCATCTGCACGTGTAGGCTGGAGCTGCTTC
aceAredr TATCTGTAGGCCCGGTAAGCGCAGCGCCACCGGGCATCAACATATGAATATCCTCCTTAG
gltAredf TCCGGCAGTCTTAAGCAATAAGGCGCTAAGGAGACCGTAAGTGTAGGCTGGAGCTGCTTC
gltAredr GTACCGGATGGCGAGGGTTGCGTCGCCATCCGGTTGTCAACATATGAATATCCTCCTTAG
icdAredf TGACAGACGAGCAAACCAGAAGCGCTCGAAGGAGAGGTGAGTGTAGGCTGGAGCTGCTTC
icdAredr GACGTTAAGTCCCCGTTTTTGTTTTTAACAATTATCGTTACATATGAATATCCTCCTTAG
mdhredf GCAATAGACACTTAGCTAATCATATAATAAGGAGTTTAGGGTGTAGGCTGGAGCTGCTTC
mdhredr AGAAGCCGGAGCAAAAGCCCCGGCATCGGGCAGGAACAGCCATATGAATATCCTCCTTAG
sucAredf AGAGTATTAAATAAGCAGAAAAGATGCTTAAGGGATCACGGTGTAGGCTGGAGCTGCTTC
sucBredr CCTTATCCGGCCTACAGGTAGCAGGTGATGCTCTTGCTGACATATGAATATCCTCCTTAG
sucCredf GGTCTAAAGATAACGATTACCTGAAGGATGGACAGAACACGTGTAGGCTGGAGCTGCTTC
sucDredr GAAAACGGACATTTATCTGTTCCCGCAGGAACAGCGAGTTCATATGAATATCCTCCTTAG
sdhCredf GTCTTAAGGGAATAATAAGAACAGCATGTGGGCGTTATTCGTGTAGGCTGGAGCTGCTTC
sdhBredr ATAAGACTGTACGTCGCCATCCGGCAACCACTACAACTACCATATGAATATCCTCCTTAG