Figure 1. Spectrophotometrical analyses.

Spectrophotometrical analysis of the incubation mixture containing (A) 50 µM monoHER, 1.6 nM horseradish peroxidase (HRP) and 33 µM H₂O₂. The same experiment was carried out in (B) the presence of 40 µM GSH, (C) 40 µM ascorbate and (D) both 40 µM GSH and 40 µM ascorbate. The UV/Vis scans were recorded 30, 150 and 300 seconds after the addition of HRP. A typical example is shown.