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. Author manuscript; available in PMC: 2011 Aug 18.
Published in final edited form as: Glycoconj J. 2010 Aug 18;27(6):625–633. doi: 10.1007/s10719-010-9306-1

FIGURE 4.

FIGURE 4

A. GAG chains, primed by xyloside 5 in endothelial cell line, were analyzed using anion exchange HPLC. The bound GAG chains were eluted with a linear gradient of 0.2 M to 1 M NaCl over 80 minutes at a flow rate of 1ml/min. The radiodetector trace of the GAG chains isolated from control cells (grey trace) and cells treated with 100 μM xyloside (black trace) are shown. B. GAG chains, primed by xyloside 5 in endothelial cell line, were analyzed using two SEC (3000 SWXL) that were connected in tandem and were eluted with an isocratic gradient of phosphate buffer for 90 minutes at a flow rate of 0.5 ml/min as described in the experimental section. The elution profile of the GAG chains, primed by xyloside 5 at 100 μM (black trace; control as grey trace), is shown. Vo and Vt represent void and total volume, respectively. These elution profiles are representative of two independent experiments.