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. Author manuscript; available in PMC: 2010 Nov 8.

Published in final edited form as: Cancer Gene Ther. 2007 Dec 14;15(2):115–125. doi: 10.1038/sj.cgt.7701110

(a) Schematic representation of the two oncolytic VV, vvDD and vvDD-CD, utilized in the present study. Presented is the schematic representation of deletions of two viral genes for TK and VGF and the insertion of the transgenes for enhanced green fluorescent protein (eGFP) or Saccharomyces cerevisiae fcy1 gene encoding the cytosine deaminase (CD) at the tk locus in the viral genome. In the virus vvDD-CD, the lacZ genes at the vgf loci have been inactivated due to the negative selection. (b). The new virus vvDD-CD expresses high CD activity in the virus-infected MOSEC cells. MOSEC cancer cells grown in 6-well plates were mock-infected or infected with vvDD or vvDD-CD at the indicated MOI. At 24 hr postinfection, cells were harvested and extracts were made with 250 ul of lysis buffer. CD enzymatic activity in the cell extracts was measured. The values are the mean +/− SD from the triplicates.

(a) Schematic representation of the two oncolytic VV, vvDD and vvDD-CD, utilized in the present study. Presented is the schematic representation of deletions of two viral genes for TK and VGF and the insertion of the transgenes for enhanced green fluorescent protein (eGFP) or Saccharomyces cerevisiae fcy1 gene encoding the cytosine deaminase (CD) at the tk locus in the viral genome. In the virus vvDD-CD, the lacZ genes at the vgf loci have been inactivated due to the negative selection. (b). The new virus vvDD-CD expresses high CD activity in the virus-infected MOSEC cells. MOSEC cancer cells grown in 6-well plates were mock-infected or infected with vvDD or vvDD-CD at the indicated MOI. At 24 hr postinfection, cells were harvested and extracts were made with 250 ul of lysis buffer. CD enzymatic activity in the cell extracts was measured. The values are the mean +/− SD from the triplicates.