Fig. 5.

Activation of LXR leads to lipid accumulation in RWPE1, THP1, and HepG2 cells. A: RWPE1 cells were treated with T0901317 (1 and 3 μM) and GW3965 (2 μM). After two days of incubation, cells were fixed and intracellular triacylglycerides were visualized with Oil Red O staining (see Materials and Methods). B: THP1, HepG2, HeLa, and HEK293 cells were treated with T0901317 and subjected to Oil Red O staining. Attachment of THP1 cells to the culture dish was induced by the treatment of phorbol 12-myristate 13-acetate (PMA, 0.1 μM) for 12 h. Accumulated lipid amount was quantified by ImageJ software. GW, GW3965; LXR, liver X receptor; T, T0901317.