Fig. 2.
Serum IL-6 level and PON arylesterase activity in apoE−/− mice. A: The serum IL-6 levels at 24 h after the last injection. Mouse IL-6 concentration was determined with mouse IL-6 ELISA kit. The serum IL-6 concentrations increased 24 h after injection with rHDLwt and rHDLmilano complexes, whereas rHDL74 treatment only exhibited a level similar to the saline treatment group. Data are expressed as mean ± SD. ★P < 0.01 versus rHDLwt; †P < 0.05 and §P < 0.01versus rHDLmilano. B: Arylesterase activity. Serum was added to 1 ml of the 1 mM phenylacetate-containing solution (20 mM Tris-HCl/0.9 mM CaCl2, pH 8.0). A PON-1 activity of 1 U/l is equal to 1 mmol of phenylacetate hydrolyzed/min; arylesterase activity was expressed as units per mg serum proteins. rHDL, recombinant HDL; IL-6, interleukin-6; PON, paraoxonase.