Fig. 2.

Western blots of apoA-I (A), apoJ (B) and apoF (C). A: LDL(+) (20 μg apoB/lane), LDL(−) (20 μg apoB/lane) and HDL (1 μg apoA-I/lane) were submitted to GGE electrophoresis, and Western blot was performed as described in “Methods.” Right: Sudan Black staining (lipid staining). Left: anti apoA-I Western blot. B, C: LDL(+) (20 μg apoB/lane) and LDL(−) (20 μg apoB/lane) were submitted to SDS-PAGE in 12% acrylamide gels, and Western blots were performed as described in “Methods.” apo, apolipoprotein; GGE, non-denaturing gradient gel electrophoresis; LDL(+), electropositive low-density lipoprotein; LDL(−), electronegative low-density lipoprotein; Mw St, molecular weight standard.