Fig. 6.
Effect of incubation with donor vesicles and meβ-CD on cell viability. BHK21 cells were incubated for 1 h with 0.5 mM donor vesicles composed of cholesterol, POPC, and 14:1/14:1-PS (10:9:1, mol/mol) in the presence of indicated concentrations of meβ-CD. The cells were then washed and chased for 2 h in DMEM. Alamar Blue was added and the cells were incubated for 3 h. The cells were then counted, an aliquot of the incubation media was collected, and fluorescence was measured as specified in Materials and Methods. CTRL, untreated cells; DMEM, media containing 10% Alamar Blue, CuSO4, cells killed with CuSO4, ETOH, cells killed with ethanol. A: Total fluorescence intensity and (B) fluorescence intensity divided by cell number. Data are means of two independent experiments with three parallel samples ± SD.