Fig. 1.

Illustration of the glycerol-3-phosphate pathway. [¹³C₁₈]oleic acid is sequentially incorporated into the glycerol backbone to form triglyceride (TG). The asterisks indicate the positions of stable ¹³C isotope incorporated from the [¹³C₁₈]oleic acid precursor [¹³C₁₈]oleoyl-CoA (H₃₃C₁₇CO-SCoA). The enzymes involved are glycerol-3-phosphate acyltransferase (GPAT), acylglycerophosphate acyltransferase (AGPAT), phosphatidic acid phosphohydrolase (PAP), and diacylglycerol acyltransferase (DGAT).