Fig. 3.

Time course of [¹³C₁₈]oleoyl-incorporated triolein. HEK293 cells were incubated with [¹³C₁₈]oleic acid, precomplexed to FA-free BSA at a concentration of 150 μM or 300 μM for indicated times. Lipids were extracted and analyzed by LC/MS/MS. A: Triolein containing three [¹³C₁₈]oleoyl side chains. B: Triolein containing two [¹³C₁₈]oleoyl side chains. C: Triolein containing one [¹³C₁₈]oleoyl side chain. D: Concentration-dependent linear response of triolein. All mass units are relative to an internal standard. Values represent the mean of three data points ± SEM.