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A2AR KO mice exhibited increased PCNA-positive cells in pulmonary resistance vessels. Expression of α-SMA in pulmonary resistance vessels was determined by immunohistochemistry as described in Materials and Methods. a, b Representative photomicrographs of PCNA staining in lung. ×200. c Quantitative analysis of PCNA-positive cells in lung sections of WT andA2AR KO mice. ∗ p < 0.05, A2AR KO vs. WT, one-way ANOVA, LSD test, n = 6–7.
