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A2AR KO mice displayed no histological features of hypertensive nephropathy. Expression of α-SMA in renal arteries was determined by immunohistochemistry as described in Materials and Methods. a, b Representative photomicrographs of α-SMA staining in kidney. ×200. c Quantitative analysis of α-SMA-immunoreactivity (optical density) in WT and A2AR KO mice. WT (n = 5), A2AR KO (n = 7). p > 0.05, one-way ANOVA, LSD test.
