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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Int J Biochem Cell Biol. 2010 Sep 25;42(12):2047–2055. doi: 10.1016/j.biocel.2010.09.009

Fig. 5. Multipotent differentiation of hLBSC.

Fig. 5

(A1-3) Adipogenic differentiation of hLBSC. By 14 days in adipose induction media, 90% cells had abundant droplets which stained positively by Oil-red-O, a fat specific dye (A2), while hLBSC without adipocytic induction were negatively stained (A1). And expression of PPAR-γ2 (351bp) was detected at 0 days (lane 1), 10 days (lane 2), and 14 days (lane 3) by RT-PCR (A3), with β-actin (516bp) as control. M: pUC Mix Marker. (B1-4) Osteogenic differentiation of hLBSC. Under osteogenic induction conditions, most of the cells acquired cuboidal or polygonal shape, accompanied by the appearance of calcification spots with high refraction ratio (B1). Thirty percent induced cells were positive for AP staining (B4), with untreated hLBSC as control (B3). Expression of type I collagen (299bp) was detected by RT-PCR (B2). M: pUC Mix Marker; Lane 1: control samples; Lane 2: samples with osteogenic induction. RT (−): mRNA sample without reverse transcription. (C1-3) Neural differentiation of hLBSC. In the presence of β-mercaptoethanol, hLBSC underwent morphological transformation with appearance of long processes (C2), with untreated hLBSC as control (C1). Enhanced expression of nestin (718bp) was detected by RT-PCR (C3). M: pUC Mix Marker; Lane 1: mRNA sample without reverse transcription. Lane 2: control samples without BME treatment; Lane 2: samples with BME treatment.