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. Author manuscript; available in PMC: 2011 Sep 22.
Published in final edited form as: Biochim Biophys Acta. 2010 Sep 22;1799(9):622–629. doi: 10.1016/j.bbagrm.2010.09.003

Figure 3. Per1 knockdown inhibits αENaC protein expression.

Figure 3

A. mpkCCDc14 cells were transfected with a non-target control siRNA or a Per1 specific siRNA (Per1–8). Twenty four hours later, cells were treated with 1 μM aldosterone (aldo). Total cell lysates were collected 24 hr later and Western blot analysis was performed using an anti-αENaC antibody (gift of Dr. Carolyn Ecelbarger). An anti-actin antibody (Sigma) was used for a loading control. B. Densitometry analysis (Kodak Imaging) was performed on Western blots to determine the % decrease in αENaC protein following Per1 knockdown. Signal intensities were normalized against actin. *p<0.05 versus Non-target + aldo, n=4 in two independent experiments.