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Immunofluorescence analysis of IR-induced PCNA complexes in the interphase nuclei of normal and AT cells. The normal (NHDF and WI38) and AT (GM5823C and GM2052C) cells in G1 phase were exposed to 5 Gy of γ-rays and incubated for 30 min. The cells were extracted with hypotonic buffer I and fixed in acetone:methanol. The cells were indirectly immunolabeled for insoluble PCNA as described before.
