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Western blot analysis of insoluble proteins isolated from NHDF cells exposed to increasing doses of γ-rays (A). NHDF cells in G1 phase were irradiated (0, 5,10, 20 and 30 Gy) and the insoluble proteins were extracted after 30 min. The proteins were size fractionated on 4–20% SDS–PAGE, transferred to PVDF membrane and probed with antibody to PCNA. The signal was detected by the ECL method. (B) Histogram showing the PCNA band intensity in the insoluble proteins.
